Hoechst for live imaging
NettetImaging of Cellular Fluorescence on ... cells were stained live with 5 µM Calcein AM (Corning Cat. No. 354216), ... CMTMRos (Thermo Fisher Cat. No. M7510), and 10 µg/mL Hoechst 33342 (Thermo Fisher Cat. No. 62249) in Hank’s Balanced Salt Solution with calcium and magnesium (HBSS; Corning Cat. No. 21-023-CMR) for 45 minutes in a … NettetWe offer a number of nucleic acid stains—with various emission wavelengths—that can be used as alternatives to DAPI for imaging live cells. Nuclear counterstaining in live cells. For live-cell staining, Hoechst 33342 dye is a popular cell-permeant nuclear counterstain.
Hoechst for live imaging
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NettetHoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered all their … Nettet(for excitation of QDot, wtGFP, Hoechst Blue and DAPI) 9 . Epi White Light Excitation . Turns “ON” or “OFF” the visible overhead white lighting (for focusing in live preview and opaque colony plates) 10 . UV Transilluminator . Controls the intensity of the 300 nm UV transilluminator; (use high intensities for imaging and lower ...
NettetThermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular … Nettet1. okt. 2015 · The ideal DNA stain for live-cell imaging would (i) show high selectivity, (ii) show minimal toxicity, (iii) be fluorogenic for wash-free imaging, (iv) be applicable in …
NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used … Nettet28. apr. 2014 · Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, …
NettetHoechst Stains At-A-Glance. Non-intercalating fluorescent dye that binds to adenine-thymine rich sequences in the minor groove of dsDNA. In solution, Hoechst dyes exhibit minimal fluorescence, but upon binding …
Nettet14. aug. 2024 · Modulation of the chromatin density of living NIH/3T3 cells stained with Hoechst 34580 and evaluation by fluorescence lifetime imaging microscopy (FLIM): (a) Intensity (left) and color coded lifetime images (scale ranging from 1225 to 1420 ps) of controls (upper row), upon treatment with histone deacetylase inhibitor, VPA, for 24 h … birchwood care center mnNettetEdU Imaging Kits (Cy5) Catalog No. K1076. EdU Imaging Kits (Cy5) is a sensitive and reliable way to detect and quantify cell proliferation in cells. It is optimized for fluorescence microscopy. It is a better alternative to traditional BrdU (bromodeoxyuridine). 跳到结尾的图 … birch wood candle holdersNettetHoechst33342 is cell permeable and usually used in live cell imaging. However for long term imaging, the UV laser illumination creates a lot of photo-toxicity. Its recommended … birchwood care center ohioNettet10. jul. 2012 · Using this model, we have previously shown with a live imaging approach that HRAS G12V (hereafter referred to as V12RAS)-expressing transformed cells induce a robust inflammatory response immediately after their emergence in host skin [ 14 ]. dallas stars scouting staffNettetTraditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging. The BioTracker 650 Red Nuclear Dye is a cell-membrane permeable red fluorescent DNA dye that specifically stains nuclei in live or fixed cells. dallas stars schedule pdfNettet5. mar. 2024 · 3D pRESOLFT microscopy is fast and it requires relatively low illumination intensities, making this technology ideal for live cell imaging. However, “there is always something we can improve,”... birchwood care home easingtonNettetA camera-based confocal system can be a better solution for live imaging without compromising cell viability due to photobleaching or phototoxicity. New developments … dallas stars schedule excel