Gapdh and western blot
WebMar 20, 2024 · GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. GAPDH is highly conserved across species. For instance, human GAPDH with 335 amino acids shares about 70% identity with its 422 amino acids homolog from Arabidopsis thaliana. It is present in the cytosol, nucleus, perinuclear regions, and … WebAnti-GAPDH antibody produced in rabbit (Anti-38-kD component ); ~ 1mg/mL, affinity isolated antibody, buffered aqueous solution; Suitable for western blot; The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13; The enzyme glyceraldehyde-3-phosphate dehydrogena ... It is also suitable for …
Gapdh and western blot
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WebWestern blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 0.05 µg/mL of Mouse … WebGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible …
WebNov 30, 2024 · Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-GAPDH Antibody (0411) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) … WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品名称. 包装. AG0122. GAPDH Rabbit Monoclonal Antibody. 50µl. AZ050. Western一抗稀释液.
WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产 … WebWestern blot - Anti-GAPDH antibody - Loading Control (ab9485) This image is a courtesy of Anonymous Abreview. All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution Lane 1 : Lysate prepared from human Huh-7 cells at 2 µg
WebAnti-GAPDH Antibody (6C5) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence). Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies. Tissue extracts, nuclear extracts …
WebJul 5, 2024 · Representative western blots of the expression of β-F1-ATPase, Hsp60 and GAPDH in two different preparations (lanes 1-2) of (A) 2DG-treated (SM) and (B) OL-treated (G) cells when compared to non ... how is salidumay describeWebWestern Blotting Protocol. For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody product webpage for recommended antibody dilution. A. Solutions and Reagents how is saline manufacturedWebHorizontal lines represent mean with SD. (B) Relative protein expression level of DCLK1/GAPDH detected by Western blot was markedly increased in PCa tissues. (C) Representative Western blot of DCLK1 protein in five paired PCa (C) and BPH (N) tissues. Data are presented as the mean ± SD; ***P<0.001. how is salicylic acid madeWebGAPDH Antibody Western Blot, validation (1:500 dilution) in Mouse testis (Cat no:10494-1-AP) FH. Prasanth (Verified Customer) (08-19-2024) The band is so clear, and very specific band with almost no background noise for GAPDH. Applications: Western Blot, Primary Antibody Dilution: 1:1000; how is saline usedWebThe 293T cell line was transfected with the plasmids indicated for 48 h, and the abundance of PRL-3 and GAPDH was estimated by Western blots. Data shown are representative of three independent ... how is sale to list ratio calculatedWebJun 6, 2016 · The level of phosphorylation is measured by the level of phosphorylated GAPDH (p) divided by the total level of GAPDH. C and D, Western blot showing the level of GAPDH in heart extracts after ischemia and reperfusion in the presence of control (TAT, 1 μ m) or ψGAPDH peptide (1 μ m) (n = 4 hearts per treatment). The level of GAPDH was ... how is saliva collected as evidenceWebAll lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1 µg/ml Lane 1 : HeLa Whole Cell Lysate Lane 2 : NIH 3T3 Whole Cell Lysate Lane 3 : PC12 … how is salinity caused