WebJul 10, 2024 · CHO cell clustering assay for PT-neutralizing antibodies. The CHO cell clustering assay was used to evaluate PT-neutralizing antibodies [4 cells/50 μL/well) that had been cultured for 4 h with Ham’s F12 medium containing 10% fetal calf serum in 96-well tissue culture plates. The plates were incubated for 22–24 h at 37°C in a 5% CO 2 ... WebProperties. Description. The HCN2 gene encodes the pore-forming subunit of hyperpolarized gated, cyclic nucleotide sensitive, non-selective cation channel.The HCN2 channel expressed in the heart contributes to the pacemaker current (Ih). Creative Biolabs offers custom functional assay services. Our ready-to-use cell models are also available ...
Reporter cell lines for detection of pertussis toxin in acellular ...
WebJul 21, 2024 · For the transactivation assay, the Jurkat cells were then co-cultured with CHO-K1 cells expressing PD-L1 (CHO-PD-L1) at a concentration of 0.45 × 10 6 cells/mL in 50 mL medium (Jurkat to CHO ... WebJan 1, 2003 · Efficacy of CHO cell clustering assay was, accordingly, evaluated using purified PT treated with graded concentrations of formaldehyde at 37 degrees C for 24h. The formaldehyde-treated PT was ... dr. arshad iqbal 4519 post road
CHO Cell Line - an overview ScienceDirect Topics
Webin 2016, and “Calibration of pertussis toxin BRP batch 1 in a standardised CHO cell-based clustering assay” published in Pharmeureuropa Bio & Scientific Notes in 2016). The specific CHO assay described in the general chapter is intended only for the testing of non-adsorbed purified pertussis components. WebThe β-Arrestin cell line is a stable clonal cell line that provides robust responses to more than 90% of all known G protein-coupled receptors (GPCRs) and has accurate pharmacology, thus accelerating drug discovery and development . This assay measures an essential pathway of GPCR activation, that is, β-arrestin is recruited to activated ... WebCell-based toxicity assays can be made compatible for aluminum salt-adjuvanted vaccines by using semi-permeable transwell inserts to prevent direct contact between the cells and aluminum salts, as demonstrated by the CHO cell-clustering assay used to test for residual toxicity of pertussis vaccines [Citation 47]. dr arshad hussain